the concept of CRISPR/Cas-based selective antimicrobials was first developed and demonstrated in 2014. Vectors coding Cas9 and guide RNAs targeting genomic loci of a specific bacterial strain ...

In bacteria, CRISPR serves as a memory immune response ... that sequence produces a guide RNA that directs an enzyme called Cas9 to snip the viral DNA, preventing infection.

Various types of endonucleases – enzymes that can cut DNA – were already known before CRISPR-Cas9. The discovery of restriction enzymes in the early 1970s heralded a new age in molecular biology.

When the CRISPR Cas9 protein is added to a cell along with ... Cas proteins are used by bacteria to destroy viral DNA. They add bits of viral DNA to their own genome to guide the Cas proteins ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

coli -- a bacteria commonly used as a research tool ... artificial intelligence and bioengineering to improve how CRISPR Cas9 genome editing tools work on organisms like microbes that can be ...

Like the human immune system, bacteria learn from past infections ... introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several ...

WIRED is where tomorrow is realized. It is the essential source of information and ideas that make sense of a world in constant transformation. The WIRED conversation illuminates how technology is ...

Microorganisms produce a wide variety of natural products that can be used as active ingredients to treat diseases such as infections or cancer. The blueprints for these molecules can be found in the ...

Among the most exciting breakthroughs are gene therapy and gene editing, with CRISPR-Cas9 leading the charge. This remarkable tool, inspired by bacteria's natural defenses, acts like precise ...