Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...

Calcium phosphate–mediated transfection also proved particularly ... which not only increased the range of cell lines for which this method was suitable but also enabled its ultimate ...