很多疾病的产生是源自RNA。 这种最新发现的Cas9来自脑膜炎细菌，这种细菌每年会引发一些最严重和致命的脑膜炎病例。 研究人员利用他们新发现的NmeCas9构建了新型CRISPR系统，而且NmeCas9比CRISPR中使用的其他Cas9蛋白质小得多，更有利于临床上使用。 张燕与文章一 ...

Analysis of CRISPR/Cas9 edited cells is done to check the efficacy of the system in introducing mutations (insertions, deletions, or substitutions) in the DNA sequences of the edited cell population.

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

CRISPR-Cas9 is not the first method available to scientists for modifying DNA; it is by far, however, the easiest to use. With CRISPR-Cas9, the crRNA/tracrRNA sequence or an artificial guide RNA ...

试图了解CRISPR-Cas9系统起源的研究人员发现了一类由转座子编码的RNA引导的核酸酶，他们称其为“OMEGA”，后者可在人类细胞中用于基因组编辑。

This new tool, known as minimal, versatile genetic perturbation technology (mvGPT), combines the powers of gene editing, ...

Its primary component, the Cas9 enzyme (orange), cuts genomic DNA (blue). The enzyme is directed to its target--essentially any sequence along the genome--by hitching it to a strand of guide RNA ...

Scientists realized that they could edit and disrupt gene function in eukaryotes by engineering customizable short guide RNA to direct the enzymes to introduce cuts at specific points in the genome.

The Cas9 protein is the most widely used by scientists. This protein can easily be programmed to find and bind to almost any desired target sequence, simply by giving it a piece of RNA to guide it ...

To investigate how Cas9 gene editing affected T cells, the researchers targeted the first exon of the TRAC locus, which encodes the T cell receptor on chromosome 14. As expected, DNA sequencing ...