大家都知道，CRISPR/Cas9基因编辑技术在高效获得基因敲除突变（knockout）方面是非常有效的，但是想要通过同源重组修复途径 ...

虽然但是，还有整整75年，不知道还有多少新奇玩意会被突破。 但就这20几年，至少有几项我感觉是跑不掉"名留青史"的。 1.人类基因组（Human Genome Project，2000） ...

所设计的HDR供体模板的同源臂序列为GAPDH外显子8的末端序列，AcGFP1与GAPDH处于同一阅读框（图A）。通过流式细胞术比较分析以dsDNA和ssDNA分别作为修复模板的实验结果（图B）。结果显示，当以dsDNA作为修复模板时，即使没有Cas9 RNPs，仍然出现相当多的带有绿色荧光 ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

(B, C) Confocal microscopic images of coronal brain sections of the hippocampus of Cas9 mice, showing the EGFP ... Single AAV Systems for HDR-Mediated Genome Editing in Vivo Dual AAV Systems ...

The CRISPR-Cas9 molecular complex ... It may be possible to avert the danger by using not just one molecule to promote HDR in the future but a cocktail of different substances.

Advances in CRISPR technologies promise to accelerate the burgeoning pipeline of gene-editing therapies and broaden access to these disease-altering therapies.

Cas9, the first Cas protein repurposed for cutting DNA, introduces double-strand breaks that recruit DNA repair machinery. Despite its successes, the CRISPR-Cas9 system has several limitations, ...