Conditional gene targeting in mice allows the introduction of targeted mutations in a cell type–specific and/or inducible way. This is most commonly achieved by flanking a target gene segment ...

We have used powerful conditional “gene knockout” techniques involving the cre/lox recombinase system to create mice that lack neurotrophic factors only in a specific tissue. This allowed us to ...

Without the second gene copy to worry about, we can generate these knockout cell lines in a fairly streamlined and convenient fashion — and we can hit pretty much any gene in the genome.

Both gene knockout and knock-in models are powerful tools widely used in biomedical research to gain important insights into ...