All the knockout ES-cell lines would be archived and freely distributed to the research community. A portion of the ES-cell lines would be used to create live mice through germline transmission ...

Transgenic and Knockout Core personnel can inject gene-trapped mouse embryonic stem cells into blastocysts, with the resulting chimeras then bred to germline transmission of the desired mutation. This ...

Background Heterozygous germline mutations in the gene encoding the bone morphogenetic protein type ... In addition, we crossed this animal with Smad1± knockout mice to determine the effect of ...

Importantly, the germline knockout did not exhibit a markedly different phenotype from the B ... We sought to understand the function of OX40L using CD4+ T cell and B cell conditional knockout mice.

These gene expression changes became more robust over time and mirrored those of the Mecp2 germline knockout mice. "These data revealed a molecular cascade that drives disease independent of any d ...

In addition, this discovery stimulated the search for embryonic stem cells and ultimately led to the development of the "knock-out mouse?. In 1981 ... and one year later reported generation of giant ...

To understand the relevance of this 'gut-germline' axis to traits inherited by offspring, the scientists mated either untreated or dysbiotic males with untreated females. Mouse pups sired by ...

Methods The activation status of Nrf2 was assessed in synovial tissue from patients with RA using immunohistochemistry. Antibody-induced arthritis (AIA) was induced in Nrf2-knockout and Nrf2-wild-type ...