plasmid DNA is often purified on a smaller scale for sequencing and other purposes. With this in mind, we have developed a scaled-down mini-prep protocol based on compaction precipitation.

confirming the exact sequence of the plasmid-embedded gene and its purified mRNA product. Sanger sequencing data must undergo a multi-step process to convert raw data into a usable state.

The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the size is the entire plasmid, vector plus insert. For plasmid ...

Properties such as GC-content, repeats such as poly(A)-tails and loop structures affect sequencing reactions, so any information you can provide will help us select suitable reaction protocol.

rustigianii carries a cytolethal distending toxin virulence gene on its plasmid. Elimination of this gene did not reduce the bacteria's virulence, however. This time, the whole genome sequencing ...

Further analyses revealed that the plasmid occurred almost exclusively ... researchers were able to detect the characteristic ...