2). The ratio of plasmid DNA to transfection agent within polyplexes had a major impact on transfection efficiency. Therefore, each expression vector needs a fine tuning of transfection conditions ...

Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...

With CRISPR-based gene editing gaining momentum, transfection is an essential technique in the modern laboratory. Hear from Polyplus Transfection about the challenges of transfection, and their unique ...

Production of adenovirus vectors can begin at 1 of 3 steps. Service can be initiated at the cloning stage where we clone your transgene into the adenovirus backbone plasmid, at the transfection stage ...

Reporter genes are generally joined to a regulatory DNA sequence in an expression vector that is usually propagated in Escherichia coli before transfection into the cell type of interest. 1 A control ...