The kits contain clear and unambiguous protocols, all required buffers and desalting/buffer-exchange columns for purification of His-tagged proteins in less than 50 min using the Midi spin columns ...

Figure 1: RPE65 immunocytochemistry in canine RPE cells and retinal ... manufacturer's protocol. cDNA was amplified from total RNA using RNA PCR kit (Perkin Elmer) and the conditions listed above ...