It is analogous to Southern blotting, which is used to analyze DNA. This protocol describes the transfer of RNA from agarose gels to nylon membranes and the fixation of the RNA to the membrane 1,2,3.
The virus uses its own RNA copying machine, called a polymerase, to make duplicates of RNA inside the vesicles. Some of the copies are utilized to make more viral proteins, such as the spike.
In a paper published today (August 15) in PNAS, scientists from the Scripps Research Institute in La Jolla, California, report on their use of in vitro evolution to generate an improved polymerase ...
tRNAs have a distinct cloverleaf secondary structure and an L-shaped tertiary structure. The cloverleaf structure is formed by the folding of the single-stranded tRNA molecule, which is typically ...