A CRISPR knockout (CRISPRko), therefore ... “RNAi allows for reversible gene silencing, unlike most applications of CRISPR/Cas9 where edits to the genome are permanent,” says Lin.

Both gene knockout and knock-in models are powerful tools widely used in biomedical research to gain important insights into ...

To create a knockout mouse, scientists genetically engineer the animal by disrupting a gene of interest. This may be done by deleting portions of the gene's DNA sequence to make it nonfunctional ...

similar to the STK33 knockout mice and the men with the STK33 mutation,” Courtney Sutton, another co-author of the study, said. “Importantly, the contraceptive effect was reversible.