PCR was developed in 1983 ... “These assays can be used to validate whether a siRNA is efficiently transfected into cells, as well as monitor the molecule's lifespan,” says McDonald.
Ambion公司可为您提供siRNA的序列,您可选择化学合成siRNA ... 利用Northern或者RPA的方法检测miRNA;您也可使用定量RT-PCR来检测定量miRNA,所有的引物已经为您准备好了;您可使用miRNA抑制剂和miRNA模拟剂来精确研究您感兴趣的miRNA的功能。
Either accession numbers from GenBank or raw sequences or siRNA sequences can be used to generate hairpin PCR primers. Complete the design of the template oligonucleotide by incorporating miR-30 ...