We describe here a detailed protocol for generating gene knockout rats by homologous recombination in embryonic stem (ES) cells. This protocol comprises the following procedures: derivation and ...

Although the use of homologous recombination in stem cells currently lags behind the use of zinc fingers for creating knockout rats, it might allow researchers to reliably introduce genes into ...

Homologous recombination plays essential roles in the repair of DNA double strand breaks (DSBs) and collapsed replication forks. The fundamental reaction in homologous recombination is the exchange of ...

Gene knockout models involve the deliberate ... or nonhomologous end-joining (NHEJ). Historically, homologous recombination ...

The Transgenic and Knockout Core can develop mice with targeted mutations in the entire mouse or in specific tissues or cell types. It normally takes about a year to generate a gene-targeted mouse.

Our emphasis is on repair of DSBs by homologous recombination, with a particular interest in the role of homologous recombination in maintaining genetic stability. Understanding the repair of DSBs is ...

Dr. Hussain and colleagues presented analysis of the PROfound study, a randomized phase III biomarker driven trial in mCRPC of olaparib in patients harboring alterations in selected DNA damage repair ...

The investigation involved a comprehensive approach, including the generation of a Setd2-CD knockin mouse model through homologous recombination ... genes in both Setd2-CD and Setd2 knockout ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Homologous recombination (HR) is important for ...