plasmid DNA is often purified on a smaller scale for sequencing and other purposes. With this in mind, we have developed a scaled-down mini-prep protocol based on compaction precipitation.

confirming the exact sequence of the plasmid-embedded gene and its purified mRNA product. Sanger sequencing data must undergo a multi-step process to convert raw data into a usable state.

The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the size is the entire plasmid, vector plus insert. For plasmid ...

This repository supplements our paper: Wick RR, Judd LM, Wyres KL, Holt KE. Recovery of small plasmid sequences via Oxford Nanopore sequencing. Microbial Genomics ...

Step-by-step instructions for creating a custom kraken 2 database that re-annotates plasmid sequences to a generic plasmid ID. First, must follow instructions from Kraken manual to download desired ...

rustigianii carries a cytolethal distending toxin virulence gene on its plasmid. Elimination of this gene did not reduce the bacteria's virulence, however. This time, the whole genome sequencing ...

Properties such as GC-content, repeats such as poly(A)-tails and loop structures affect sequencing reactions, so any information you can provide will help us select suitable reaction protocol.

Oxford Nanopore said on Tuesday that it has struck a new strategic collaboration to advance plasmid sequencing beyond legacy methods, and an expanded multi-year, multi-million dollar global contract.

Further analyses revealed that the plasmid occurred almost exclusively ... researchers were able to detect the characteristic ...