CRISPR-Cas9 is one of the biggest discoveries of the 21st century. Since it was developed in 2012, this gene-editing tool has revolutionized biology research, making it easier to study disease and ...

Abstract: The CRISPR-Cas9 system, found across bacteria and archaea, enables efficient genome engineering in eukaryotic cells. There is a challenge that Cas9 guide RNA may cause off-target activities.