Since 2006, scientists around the world have been working together to generate a targeted knockout mutation for every gene in the mouse genome. The Knockout Mouse Project (KOMP) is providing critical tools for understanding gene function and the genetic causes of human diseases.

2024年11月1日 · Here, we report the genotypes and phenotypes of heteroplasmic MT-ND5 gene-knockout mice. These mutant mice presented damaged mitochondrial cristae in the cerebral cortex, hippocampal atrophy,...

2020年8月17日 · A knockout mouse is a laboratory mouse in which researchers have inactivated, or "knocked out," an existing gene by replacing it or disrupting it with an artificial piece of DNA.

2024年7月18日 · Design, produce and systematically phenotype a null mutation mouse line across the mouse–human orthologous genome to provide insights into gene function, disease genetics, pleiotropy and co-morbidity. KOMP2 and IMPC have developed standardized pipelines for phenotyping knockout mouse strains at embryo, early adult and late adult stages.

2013年7月18日 · Mutations in whole organisms are powerful ways of interrogating gene function in a realistic context. We describe a program, the Sanger Institute Mouse Genetics Project, that provides a step toward the aim of knocking out all genes and screening each line …

2001年10月1日 · This report describes the first mutation of a signalling molecule using knockout mice. The data unequivocally showed that p56lck is crucial for T-cell development and activation in vivo.

2025年1月22日 · To identify novel modifiers of somatic expansion, we used CRISPR–Cas9 editing in Huntington’s disease knock-in mice to enable in vivo screening of expansion-modifier candidates at scale.

Wnt signal transduction is crucial for maintaining the balance between proliferation and differentiation throughout embryogenesis and postnatal life. Here, we provide a comprehensive overview of the conventional knockout mouse studies of both the canonical and the noncanonical Wnt pathways during mammalian development.

2025年1月22日 · Triple knockdown of CD11a, CD49d, and PSGL1 in T cells reduces CAR-T cell toxicity but preserves activity against solid tumors in mice

We found that injection of RNA into the cytoplasm was the most efficient method in terms of the numbers of viable blastocyst stage embryos and full-term pups generated. This method also showed the best overall knockout efficiency. Mouse is …