2025年1月2日 · In this study, we demonstrate a fully integrated 3D-printed biosensensing device employing a CRISPR/Cas12a-based dual-enzymatic mechanism for highly sensitive and user-friendly nucleic acid detection. A plastic probe stick was designed to host small-sized gold nanoparticles, enhancing enzyme labeling density.

2023年12月20日 · To address these challenges, we developed a colorimetric, photothermal, and fluorescent triple signal-based CRISPR biosensor (CPF-CRISPR). This biosensor utilizes 3,3′,5,5′-tetramethylbenzidine (TMB) as a chromogen that can be oxidized by horseradish peroxidase (HRP) to produce oxidized TMB (oxTMB), resulting in a color change from ...

2024年8月1日 · CRISPR/Cas-powered wearables, supported by microneedles and flexible substrates, are promising for real-time and continuous on-body monitoring. Outlines challenges and perspectives in developing multiplex miniaturized biosensors using CRISPR/Cas, innovative platforms, AI, and promoting commercialization.

6 天之前 · The rapid, simple, and sensitive detection of nucleic acid biomarkers plays a significant role in clinical diagnosis. Herein, we develop a label-free and point-of-care approach for isothermal DNA detection through the trans-cleavage activity of CRISPR-Cas12 and the growth of gold nanomaterials in agarose gel. The presence of the target can activate CRISPR-Cas12a to cleave single-stranded DNA ...

2024年12月2日 · Here, we developed a machine learning-supported, dual-channel biosensor-in-microdroplet platform for meat species authenticity detection named CC-drop (CRISPR/Cas12a digital single-molecule microdroplet biosensor). This strategy allowed us to quickly identify and analyze animal-derived components in foods.

2024年5月1日 · Schematic illustration of an electrochemical biosensor developed using the CRISPR-Cas9 collateral cleavage system to detect target sequences. The sensor follows the switch-off signaling approach by using methylene blue-tagged DNA as a reporter probe and a trans-cleavage substrate for the Cas12a enzyme. (For interpretation of the references to ...

2024年6月11日 · Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-Cas system have broad applications for the detection of nucleic acids due to their strong specificity, high sensitivity, and excellent programmability.

2025年1月15日 · In this study, an electrochemical biosensor for progesterone based on SRTF and CRISPR-Cas14a was developed, which can be applied to environmental water, milk and urine samples for highly sensitive and selective detection of progesterone.

2024年1月1日 · Herein, we developed a CRISPR-empowered electrochemical biosensor by combining the advantages of CRISPR/Cas13a and primer exchange reaction (PER), named PER-E-CRISPR, for target amplification-free and sensitive detection of miR-21.

2023年6月23日 · Specifically, the methods and the techniques of integrating CRISPR-Cas into various biosensing systems, the design strategies of a CRISPR-Cas system in a biosensor, and the integration between conventional nucleic acid probe-based recognition elements and CRISPR-based recognition elements require additional research and development endeavors.