2018年12月20日 · These membraneless compartments, such as ribonucleoprotein particle granules, have been shown to coalesce into droplets with liquid-like features surrounded by dynamic intracellular environments....

2024年11月1日 · Liquid‒liquid phase separation (LLPS) has emerged as a key mechanism for organizing cellular spaces independent of membranes. Biomolecular condensates, which assemble through LLPS, exhibit...

2023年6月24日 · Membrane-remodeling coacervates could potentially play a role in artificial cell division if their location on the membrane and the force they exert on the membrane could be controlled. Studies on multicomponent GUVs showed that local phase separation in the membrane could lead to diverse shape deformations, including budding, endocytosis, and ...

2023年5月22日 · Here, we demonstrate that interactions between protein condensates - including hollow ones, and membranes can lead to remarkable morphological transformations and provide a theoretical framework to...

Biomolecular condensates are highly versatile membraneless organelles involved in a plethora of cellular processes. Recent years have witnessed growing evidence of the interaction of these droplets with membrane-bound cellular structures.

2024年4月6日 · By using GUVs containing the azo-PC photolipid as minimalistic artificial cells, we effectively generated and characterized light-induced membrane-condensate wetting transitions leading to fast reversible endocytosis (within a few seconds) over multiple photoswitching cycles.

2020年10月12日 · Numerous membraneless organelles assembled via liquid-liquid phase separation (LLPS), known as condensates, also facilitate compartmentalization of cellular functions. Emerging evidence shows that these two organelle types interact in …

2021年8月1日 · In this review, we discuss the interactions between three basic molecular assemblies that have shown promise in shaping synthetic cells: deformable membranes, phase-separated condensates, and the dynamic cytoskeleton. The interplay between these components facilitates compartmentalization, force generation, and dynamic shape changes.

In this project, we aim to create catalytic pockets in the membrane of a synthetic cell that can be controllable by light triggers to couple the membrane dynamics with the transphosphorylation of nucleic acid condensates.

2023年3月1日 · Membrane/protein-condensate interactions provide biologically relevant functionalities. Observations of membrane-reshaping behaviors of protein condensates have added new modalities to understanding membrane curvature generation in cells.