Split selectable marker systems utilizing inteins facilitate gene ...
2023年5月26日 · To split Kan R (i.e., nptII) and Hyg R (i.e., hpt), gBlocks Gene Fragments containing either 5′-Kan R /Hyg R and N-terminal of NpuDnaE or C-terminal of NpuDnaE and …
A high efficiency gene disruption strategy using a positive–negative ...
2014年11月1日 · We have developed a method for constructing split-marker gene-disruption cassettes with two selection markers (hyg and neo). The upstream linear cassette …
Split-Marker Recombination for Efficient Targeted Deletion of …
Split-marker strategy for gene deletion. (A) Primers F1/F2 and F3/F4 amplify target gene flanking sequences. Primers M13R/HY and M13F/YG amplify "HY" and "YG" marker fragments, …
Split selectable markers - Nature Communications
2019年10月31日 · By combining split points, we create 3- and 6-split Hygromycin resistance genes, demonstrating that higher-degree split markers can be generated by a “chaining” …
Targeted Gene Replacement in Fungi Using a Split-Marker …
2011年11月23日 · Split-marker is a PCR-based method that does not require subcloning and only involves two rounds of PCR. The hygromycin B phosphotransferase (hph) gene conferring …
Split Selectable Genetic Markers Enable Multi-Gene Plant …
Researchers demonstrate for the first time in plants that the split selectable marker systems split-Kan R and split-Hyg R are effective for co-transformation of multiple genes in herbaceous and …
Genetic transformation of the tomato pathogen - Springer
2014年11月21日 · A genetic transformation system based on a split-marker approach was developed and tested to knock out a P. lycopersici gene encoding for a lytic polysaccharide …
Facile methods for generating human somatic cell gene knockouts …
2004年1月16日 · Emerging evidence suggests that recombinant adeno‐associated viral (rAAV) vectors can be used for specific gene targeting in human somatic cells. We have developed an …
(A) Split-marker approach for target gene knockout in F. virguliforme.
(PDF) Genetic transformation of the tomato pathogen …
First of all a gene knockout system was needed to... Protoplasts of the pathogenic plant fungus, Crinipellis perniciosa, were transformed to hygromycin B resistance using the pAN7-1 plasmid, …