Lentiviral particles can efficiently infect a broad range of cell types, including both dividing and nondividing cells. The addition of puromycin will allow you to select cells that are stably …

The main procedures of shRNA lentiviral vector include that (1) Target sequences screening and shRNA oligonucleotides designing, (2) insert designed oligonucleotides into lentiviral vectors, …

For stable loss-of-function experiments, you should generate lentiviral particles and infect the target cells. Addition of puromycin will allow you to select for cells that stably express your …

2015年12月8日 · To explore the application of AGO2-based shRNA system in mammalian cells, we designed shRNA vectors targeting the EGFP reporter gene and evaluated the effects of …

Lentiviral particles can efficiently infect a broad range of cell types, including both dividing and non-dividing cells. Addition of puromycin will allow you to select for cells that are stably …

2006年3月24日 · In summary, we have produced a genome-scale lentiviral shRNA library to target human and mouse genes in a wide range of cell types, developed a pipeline to …

Here, we present our complete optimized workflow for lentiviral particle production starting from bacterial glycerol stocks through purification of the pLKO.1 expression plasmid containing a …

2017年2月28日 · Short hairpin RNA (shRNA) is an established and effective tool for stable knock down of gene expression. Lentiviral vectors can be used to deliver shRNAs, thereby providing …

The main procedures of shRNA lentiviral vector include that (1) Target sequences screening and shRNA oligonucleotides designing, (2) insert designed oligonucleotides into lentiviral vectors, …

The MISSION ® TRC shRNA libraries are lentiviral based shRNA vector collections for use in gene knockdown studies. This protocol describes the use of MISSION ® TRC shRNA …