Meganuclease - Wikipedia
Meganucleases are "molecular DNA scissors" that can be used to replace, eliminate or modify sequences in a highly targeted way. By modifying their recognition sequence through protein …
最早的基因编辑工具——归巢核酸内切酶(Meganuclease) - 哔哩哔哩
归巢核酸内切酶(Meganuclease)是具有较大识别位点(12至40个碱基对的双链DNA序列)特征的脱氧核糖核酸内切酶，该酶的切割位点在任何给定的基因组中通常只出现一次。
History of genome editing: From meganucleases to CRISPR
Here, we summarize the history of genome editing platforms, starting from enhanced integration of foreign DNA by meganuclease-mediated double-strand breaks to CRISPR/Cas9, the …
基因编辑的世界 - TiPLab
归巢核酸内切酶（Meganuclease） 这是一种以 较长识别片段 （具有14至40个碱基对的双链DNA序列）为特征的核酸内切酶，其中LAGLIDADG家族应用最为广泛，主要存在于真核细胞 …
Meganucleases and Their Biomedical Applications - ResearchGate
2011年1月15日 · Meganucleases are ideal scaffolds to engineer enzymes that accurately cleave DNA and induce recombination. Meganucleases can recognise unique sites of cleavage within …
579. Meganucleases as an Efficient Tool for Genome Editing
We have developed a next-generation meganuclease platform called “ARCUS” that overcomes these production difficulties and can produce nucleases with customized activity and …
兆核酸酶 - 百度百科
meganuclease 定 义 一类核酸酶内切酶，其识别序列较长，位点极为罕见，切割产生的片段很大，是定位基因操作的工具。 应用学科 生物化学与分子生物学（一级学科），酶（二级学科）
Meganucleases and Their Biomedical Applications
2011年1月17日 · Meganucleases are double-stranded DNAses that target large recognition sites. Meganucleases promote efficient gene targeting through double-strand-break-induced …
Nucleases in gene-editing technologies: past and prologue
Meganuclease, a class of deoxyribonucleases that recognizes long DNA sequences (12 to 40 base pairs) [7], was the first type of nuclease repurposed as a gene-editing tool. In the case of …
Molecular mechanisms, off‐target activities, and clinical potentials of ...
2020年5月13日 · Meganucleases in mitochondria belong to LAGLIDADG family of homing endonucleases, which recognize sites corresponded to intron-free or intein-free genes. 2 They …