Download over 2,700 carefully annotated plasmid and sequence files including commonly used cloning vectors from all major suppliers

2024年7月24日 · We have a helpful introduction to deciphering plasmid maps. Let’s start with a classic plasmid: pBR322. [1] . It is often used as a backbone for derivative vectors because it has all the features needed for successful cloning (Figure 1). From the map center, we can learn that the size of the linearized plasmid is 4361 base pairs (bp).

Plasmid are extrachromosomal, small and circular DNA molecule, which replicate independently. They are widely used as a cloning vector in recombinant DNA technology

Plasmid vectors help to introduce genes of interest into host cells to enable expression of their functions. These vectors are widely used in synthetic biology, biomedicine, gene therapy, targeted drug screening and agricultural biotechnology.

2023年8月3日 · Plasmids and bacteriophage are commonly used molecular vectors which are described as following. Plasmids are circular DNA molecule which are present independently inside the bacterial cell and has one or more genes. These genes often play an important role in the host bacterium.

A schematic representation of the pBR322 plasmid, one of the first plasmids to be used widely as a cloning vector. Shown on the plasmid diagram are the genes encoded (amp and tet for ampicillin and tetracycline resistance respectively), its origin of replication (ori), and various restriction sites (indicated in blue).

2024年5月12日 · a. Plasmid vector. Plasmids are small extrachromosomal circular DNA molecules capable of replicating autonomously within the host cell. These are also termed as the workhorse cloning vector in recombinant DNA technology. Plasmids are widely used as vectors in all three domains of life; however, these are frequently used in bacteria and yeasts.

Plasmid-cloning vectors are derived from bacterial plas mids and are the most widely used, versatile, and easily manipulated ones. The following are different types of plasmid vectors:

Plasmid map diagram online drawer. You can export the results as SVG format.

2023年6月20日 · a DNA fragment (usually isolated by PCR and/or restriction digestion) is cloned into a plasmid cut with a compatible restriction enzyme; the recombinant plasmid is transformed into bacteria; the bacteria are allowed to multiply, usually in liquid culture; a large quantity of the recombinant plasmid DNA is isolated from the bacterial culture