The ExpiCHO transient transfection systems allows for facile production of milligrams to grams of protein in CHO cells and de-risks the transition from transient to stable material during drug …

Here, we describe protocols for optimizing expression in transient expression experiments and isolating stable CHO cell lines using two types of self-made reagents, namely, lipoplexes and …

In this study, the optimal conditions for PEI transient transfection of CHO cells in order to produce rainbow trout IFN-γ recombinant protein was experimentally determined.

Recombinant protein expression in mammalian cell technology relays on efficient DNA transfer crossing the endosomal or lysosomal membrane, reaching the cytoplasm and finally entering …

Sep 2, 2019 · High cell densities for transient transfection with polyethyleneimine (PEI) can be used for rapid and maximal production of recombinant proteins. High cell densities can be …

Jan 1, 2013 · We have generated a high-yielding transient transfection platform based on suspension-adapted CHO K1 cells for generating recombinant proteins. This new platform has …

Transient transfection is a relatively low-cost, rapid method for producing recombinant proteins, but generally provides fairly low titers as expression is predominately episomal and no …

To overcome this issue, we developed an advanced transient expression system consisting in the synergistic association of a novel CHO chemically defined medium and a powerful transfection …

Jan 1, 2011 · Here, we describe protocols for optimizing expression in transient expression experiments and isolating stable CHO cell lines using two types of self-made reagents, …

2 days ago · Cell culture and transfection. CHO cells (#A11557-01; Carlsbad, CA, USA) were cultured in DMEM/F12 medium containing 10% fetal bovine serum (FBS) (ProteinEasy …