Conditional gene knockout is a technique used to eliminate a specific gene in a certain tissue, such as the liver. [1] [2] This technique is useful to study the role of individual genes in living organisms. It differs from traditional gene knockout because it targets specific genes at specific times rather than being deleted from beginning of life.

Conditional gene knockout technology allows researchers to eliminate gene expression in specific tissues at a particular point in time, in contrast to traditional knockout studies that eliminate the gene altogether. These techniques not only ensure that transgenic models avoid embryonic death from gene knockout, but also better recapitulate ...

Gene knockouts (also known as gene deletion or gene inactivation) are a widely used genetic engineering technique that involves the targeted removal or inactivation of a specific gene within an organism's genome. This can be done through a variety of methods, including homologous recombination, CRISPR-Cas9, and TALENs.

Conditional knockout (cKO) mice are genetically engineered animal models in which certain gene is inactivated in a specific tissue or on an inducible basis. Researchers can understand certain gene functions through time or tissue specific deletion to …

2019年2月26日 · Conditional gene knockout (CKO) is a technique that eliminates gene expression in a specific organ, tissue, or cell at a specific time. Credit: Tobias Arhelger/Shutterstock.com. In...

2017年1月30日 · Here, we present CRISPR–FLIP, a strategy that provides an efficient, rapid and scalable method for biallelic conditional gene knockouts in diploid or aneuploid cells, such as...

Conditional gene knockout technology allows researchers to eliminate gene expression in specific tissues at a particular point in time, in contrast to traditional knockout studies that eliminate the gene altogether.

2019年4月24日 · In this study, we developed an easy and efficient strategy to generate gene knockout-rescue systems with two episomal vectors, allowing conditional knockout of essential genes. Using this system, one plasmid encodes Cas9 and gRNA for essential gene knockout, and the other plasmid encodes the target gene controlled by Tet-Off system.

Here, we present CRISPR-FLIP, a strategy that provides an efficient, rapid, and scalable method for bi-allelic conditional gene knockouts in diploid or aneuploid cells such as pluripotent stem cells, 3D organoids and cell lines by co-delivery of CRISPR/Cas9 and a …

Here we describe a CRISPR/Cas9-based OPTimized inducible gene KnockOut method (OPTiKO) for conditional loss-of-function studies in human cells. This approach relies on an improved tetracycline-inducible system for conditional expression of single …