2023年12月8日 · Targeted Cas9 protein degradation by AcrIIA1 could modulate Cas9 nuclease activity in human therapies. The small size and specificity of AcrIIA1 could be used in a CRISPR-Cas proteolysis-targeting chimera (PROTAC), providing a tool for developing safe and precise …

2017年2月3日 · Here we report that tagging Cas9 with ubiquitin-proteasomal degradation signals can facilitate the degradation of Cas9 in non-human primate embryos.

2020年7月7日 · Here, we show that the Listeria phage protein AcrIIA1 selectively triggers degradation of catalytically active Cas9, through a direct interaction between the AcrIIA1 CTD …

2023年3月29日 · Targeted Cas9 protein degradation by AcrIIA1 could modulate Cas9 nuclease activity in human therapies. The small size and specificity of AcrIIA1 could be used in a …

In this report, we show that AcrIIA1 induces degradation of SpyCas9 and SauCas9 by direct binding in human cells. This surprising observation could be used to develop a Cas9 …

Cas9 degradation is specifically triggered by AcrIIA1 and is not a general consequence of Cas9 inhibition by any anti-CRISPR. Given that AcrIIA1 induces Cas9 degradation, we expected that …

the half-life of Cas9 can alleviate its potential neurotoxicity in the brain. Based on the fact that neurons in the adult CNS stay at the quiescent phase (G0) of a cell cycle, we tested the effect …

2020年7月8日 · Here, we show that the Listeria phage protein AcrIIA1 selectively triggers degradation of catalytically active Cas9, through a direct interaction between an unstructured …

Nonetheless, evading Keap1-mediated protein degradation leads to sustained dCas9 deposition on chromatin, leading to enhanced dCas9-mediated transcriptional regulation. These results …