2022年10月28日 · Here the authors report an optimised photoactivatable Cre recombinase system, doxycycline- and light-inducible Cre recombinase (DiLiCre), and generate a DiLiCre …

With the Tet-OFF system, you can administer doxycycline to the mice from birth until the time you want Cre activity to knockout your target gene. To summarize the Tet-inducible Cre systems, …

2022年10月28日 · Here, we engineer an optimized photoactivatable Cre recombinase system that we refer to as doxycycline- and light-inducible Cre recombinase (DiLiCre). Following extensive …

In principle, one mouse must have a tissue-specific driven cre gene and another mouse have loxP flanked (floxed) alleles of interest gene Y. Expression of Cre recombinase excises floxed loci …

2024年8月19日 · Vehicle controls can reveal potential Cre leakiness when administrated to CreER T2-positive floxed mice. Leaky Cre activity is caused by nuclear localization of …

2020年3月17日 · The Cre-loxP colony can now be maintained by breeding experimental mice (fl/fl: Cre/+) with a further homozygous floxed (fl/fl) mouse, which will produce 50% …

2006年4月1日 · The purpose of this work was to generate a transgenic mouse in which Cre production was under control of both the type II collagen promoter and doxycycline …

2023年5月1日 · We address the three main issues with skeletal Cre mouse models, namely (1) Cre specificity—avoiding Cre expression in unintended cell types; (2) Cre toxicity—reducing …

2014年7月25日 · Following doxycycline induction of cre, Sod2flox/flox cre mice demonstrated increased signs of oxidative stress in the RPE and accumulation of autofluorescent material by …

2012年11月27日 · In this report, we detail the generation and analysis of a knock-in, inducible Cre line designed to drive recombination specifically within the Rx expression domain. Crossing …