Oct 28, 2022 · Here the authors report an optimised photoactivatable Cre recombinase system, doxycycline- and light-inducible Cre recombinase (DiLiCre), and generate a DiLiCre mouse line which they use...

With the Tet-OFF system, you can administer doxycycline to the mice from birth until the time you want Cre activity to knockout your target gene. To summarize the Tet-inducible Cre systems, administration of doxycycline determines when the KO will occur, and the choice of the promoter that controls rtTA or tTA protein expression determines in ...

Oct 28, 2022 · Here, we engineer an optimized photoactivatable Cre recombinase system that we refer to as doxycycline- and light-inducible Cre recombinase (DiLiCre). Following extensive characterization in cancer cell and organoid systems, we generate a DiLiCre mouse line, and illustrated the biological applicability of DiLiCre for light-induced mutagenesis ...

In principle, one mouse must have a tissue-specific driven cre gene and another mouse have loxP flanked (floxed) alleles of interest gene Y. Expression of Cre recombinase excises floxed loci and inactivates the gene Y. Cre- loxP system is a widely used powerful technology for …

Aug 19, 2024 · Vehicle controls can reveal potential Cre leakiness when administrated to CreER T2-positive floxed mice. Leaky Cre activity is caused by nuclear localization of CreERT2 in absence of...

Mar 17, 2020 · The Cre-loxP colony can now be maintained by breeding experimental mice (fl/fl: Cre/+) with a further homozygous floxed (fl/fl) mouse, which will produce 50% heterozygous Cre, homozygous floxed (fl/fl: Cre/+) and 50% Cre negative, …

Apr 1, 2006 · The purpose of this work was to generate a transgenic mouse in which Cre production was under control of both the type II collagen promoter and doxycycline administration, and to validate its ability to produce tissue-specific and temporally selective excision of loxP flanked DNA when crossed with a floxed transgenic mouse.

May 1, 2023 · We address the three main issues with skeletal Cre mouse models, namely (1) Cre specificity—avoiding Cre expression in unintended cell types; (2) Cre toxicity—reducing the unwanted effects of Cre (beyond loxP recombination) on cellular processes and tissue health; and (3) Cre inducibility—improving the dynamic range for Cre in inducible ...

Jul 25, 2014 · Following doxycycline induction of cre, Sod2flox/flox cre mice demonstrated increased signs of oxidative stress in the RPE and accumulation of autofluorescent material by age 2 months. They showed a gradual decline in the ERG response and thinning of the outer nuclear layer (by SD-OCT), which were statistically significant by 6 months.

Nov 27, 2012 · In this report, we detail the generation and analysis of a knock-in, inducible Cre line designed to drive recombination specifically within the Rx expression domain. Crossing this line with a reporter line demonstrates that recombination can be mediated within the early optic vesicle and throughout retinal development.