Gene knockouts (also known as gene deletion or gene inactivation) are a widely used genetic engineering technique that involves the targeted removal or inactivation of a specific gene within an organism's genome. This can be done through a variety of methods, including homologous recombination, CRISPR-Cas9, and TALENs.

2023年8月23日 · By constructing knockout, complementation, and overexpression strains and comparing and assessing the differences between them, researchers can precisely explore the function of the target gene. The aim of this review is to provide relevant researchers with a reference for selecting knockout techniques.

2023年5月26日 · Here, we describe the aggregation, harmonization, and analysis of ~14,500 yeast knockout screens, which we call Yeast Phenome. Using this unique dataset, we characterized two unknown genes (YHR045W and YGL117W) and showed that tryptophan starvation is a by-product of many chemical treatments.

2024年11月15日 · Gene knockout disrupts cellular homeostasis, altering gene expression, and phenotypes. We investigated whether cells return to their pre-knockout transcriptomic state through adaptive evolution experiments on hap4Δ and ade1Δ yeast strains.

2023年12月4日 · Spot test validation and subcellular structure observation demonstrated that, following a 3-h treatment with 60 mM HMF, the SIW14 gene knockout strain exhibited a 12.68% increase in cells with abnormal endoplasmic reticulum (ER) and a 22.41% increase in the accumulation of reactive oxygen species compared to the BY4741 strain.

A wild-type E. coli K-12 MG1655 strain previously evolved under glucose minimal media at 37 °C 31 (denoted as “Ref”) was used as the starting strain in order to isolate biological changes caused by adaption to the loss of a gene product from those caused by adaption to the growth conditions of the experiment (Fig. 1e). Ref was a non ...

2018年9月18日 · Gene knockout (KO) experiments provide an established experimental approach not just for studying gene function, but also for unraveling regulatory networks in which a gene and its gene product...

2024年1月29日 · We developed FastKnock, an efficient next-generation algorithm for identifying all possible knockout strategies (with a predefined maximum number of reaction deletions) for the growth-coupled overproduction of biochemical (s) of interest.

A gene knockout is a powerful genetic tool in which a gene may be suppressed so that its function can be studied in detail. Knockout organisms are used to study gene function by investigating the effect of the lack of a gene by their phenotypic comparison …

Whole genome sequences are now available for two closely related K-12 strains, MG1655 (Blattner et al, 1997) and W3110 (Hayashi et al, 2006).