Guide RNAs are small nucleolar RNAs that, along with riboproteins, perform intracellular RNA alterations such as ribomethylation in rRNA and the introduction of pseudouridine in preribosomal RNA. [34] Guide RNAs bind to the antisense RNA sequence and regulate RNA modification.

向导RNA（guide RNA，gRNA），也称为小向导RNA（single guide RNA，sgRNA）。是作用于动质体（kinetoplastid）体内一种称为RNA编辑（RNA editing）的后转录修饰过程中。也是一种小型非编码RNA。可与pre-mRNA配对，并在其中插入一些尿嘧啶（U），产生具有作用的mRNA。

向导RNA（guide RNA，gRNA），也称为小向导RNA（small guide RNA，sgRNA）。是作用于动质体（kinetoplastid）体内一种称为RNA编辑（RNA editing）的后转录修饰过程中。也是一种小型非编码RNA。可与pre-mRNA配对，…

2020年11月5日 · The team teased out some guidelines for guide RNA design, which led to their Rule Set 1 and Rule Set 2 — quantitative models to assess sgRNA on-target specificity.

嚮導RNA （guide RNA， gRNA），也稱為 单链嚮導RNA （single guide RNA， sgRNA）。 是作用於 動質體 （kinetoplastid）體內一種稱為 RNA編輯 （RNA editing）的 後轉錄修飾 過程中。 也是一種小型 非編碼RNA。 可與pre-mRNA配對，並在其中插入一些 尿嘧啶 （U），產生具有作用的 mRNA。 Daniel E. Golden & Stephen L. Hajduk.

The Cas9 cleavage site is determined by a small RNA molecule, termed guide RNA (gRNA), that base pairs with the target DNA and at the same time binds to Cas9 to initiate the site‐specific double‐strand break (Fig. 1). What is not generally known is that the discovery of the first gRNAs and the concept that a small RNA directs a protein ...

Guide RNA refers to a RNA molecule that contains an anchor sequence and is used to direct the editing of preedited RNA by hybridizing with it and guiding the addition or deletion of nucleotides as directed by the template.

CRISPR: Guide to gRNA design. Learn the steps involved in CRISPR ranging from designing your gRNA and repair templates to approaches to verify your genome edit.

2020年10月13日 · Our database not only contains computationally determined candidate guide RNA sequences, but of even greater value, over 4000 sequences which have been functionally validated either through direct amplicon sequencing or manual curation of literature from over 1000 publications.

2025年1月24日 · In this study, we engineered a CRISPR–Cas12a system with a programmable proximity-activated guide RNA (PARC–Cas12a), providing novel insight into the integration of RNA engineering and type V CRISPR systems. A key contribution of our work is the demonstration of the successful splitting and reassembly of the …