Various gene knockout strategies have been utilised to deactivate specific genes in order to determine the effects of these genes on the function of microbes. Deactivation of genes can lead to deletion of certain proteins and functions.

2024年4月3日 · We describe a cross-species precision disease modeling platform that exploits mouse genetic diversity to bridge cell-based modeling with whole organism analysis.

2023年8月23日 · This article provides a review of current effective knockout methods for studying the function of bacterial drug resistance genes. Knockout eliminates the function of a drug resistance gene, while gene overexpression can positively verify the gene’s function. Gene complementation can theoretically revert the changes of knockout.

RNA interference (RNAi) is a cellular process involved in the silencing of genes, which makes RNAi important for observing and understanding the function of specific gene products. Short interfering RNA (siRNA) pathway is a RNAi pathway, where exogenous double stranded RNA is introduced to the cell …

In cell biology, double-stranded RNA can be injected into mammalian cell cultures to knock down the expression of its target sequence mRNA and thereby significantly decrease the levels of protein product in the cell [4]. The knockdown of p53 can be used to help understand its role in cell cycle regulation.

2024年7月15日 · Various methods can be used to knock out or knock-in genes for both in vitro and in vivo models. Ribonucleic acid (RNA) interference (RNAi), for example, is frequently used to silence target genes using small interfering RNA (siRNA) or small hairpin RNA (shRNA), the latter of which is associated with superior efficacy in knocking down genes 1.

2019年12月24日 · In this study, we created a CRISPR-Cas9 platform that can be modulated using a cell type–specific promoter and can mark knockout cells with a fluorescent protein in vitro and in vivo. We show that this system can be used as a biomedical research tool.

1 天前 · Fig. 1: Generation and validation in vitro and in vivo of the enAsCas12a knock-in transgenic mouse. Collectively, these data demonstrate our enAsCas12a model is capable of efficient gene editing ...

2023年8月17日 · Several technologies are available for gene knockout, including zinc-finger nuclease technology (ZFN), suicide plasmid vector systems, transcription activator-like effector protein nuclease technology (TALEN), Red homologous recombination technology, CRISPR/Cas, and …

Gene knockouts (also known as gene deletion or gene inactivation) are a widely used genetic engineering technique that involves the targeted removal or inactivation of a specific gene within an organism's genome. This can be done through a variety of methods, including homologous recombination, CRISPR-Cas9, and TALENs.