Maxam–Gilbert sequencing is a method of DNA sequencing developed by Allan Maxam and Walter Gilbert in 1976–1977. This method is based on nucleobase -specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites adjacent to the modified nucleotides .

这个是DNA测序原理的第二篇，Maxam-Gilbert化学法。 还是通过油管科普视频截图，原视频比较简单，同时自已也查阅了其他资料进行补充，就有了这篇文章，希望大牛莫拍。

2023年3月30日 · Maxam-Gilbert sequencing uses chemical treatment of DNA to induce precise breaks in the backbone of the molecule at known sites, followed by fragment separation and identification. The Maxam-Gilbert method was created as one of the first DNA sequencing methods and was widely utilized in the early days of DNA sequencing.

马克萨姆-吉尔伯特测序（英語： Maxam-Gilbert sequencing ）是一项由阿伦·马克萨姆与沃尔特·吉尔伯特于1976～1977年间开发的DNA测序方法。 此项方法基于：对 核鹼基 特异性地进行局部化学改性，接下来在改性 核苷酸 毗邻的位点处DNA骨架发生断裂 [ 1 ] 。

2022年9月3日 · In 1976-1977, Allan Maxam and Walter Gilbert developed a DNA sequencing method based on chemical modification of DNA and subsequent cleavage at specific bases. The method requires radioactive labelling at one end and …

2024年7月24日 · Maxam–Gilbert sequencing is a method for chemically sequencing DNA. It used to be popular but has been superseded by Sanger sequencing and next-generation sequencing methods because it is slow, is low-throughput, and uses dangerous chemicals.

Maxam-Gilbert的化学断裂法是DNA序列分析最常用的方法之一，它既适用于双链DNA，也适用于单链DNA。 它的基本原理是利用几个具有碱基专一性的化学切断反应将单个末端被32P标记的 DNA分子 进行部分切断，产生几组从标记末端到与碱基专一性反应相应的那种碱基在DNA中每个位点的长短不同的片段，这几组片段在凝胶电泳中并排着按链长被分开，对凝胶进行 放射自显影 后就可以得到代表每个碱基位置的带谱，从这个带谱可以直接读出从标记末端向另一个末端方 …

The Maxam-Gilbert sequencing approach was a significant breakthrough and become the methodology of choice, since it allowed the direct sequencing of purified DNA, without requiring previous in vivo cloning and ssDNA preparation steps.

Maxam–Gilbert Method. The Maxam–Gilbert method is a method based on chemical degradation introduced by Maxam and Gilbert in the late 1970s. The first step is to convert the DNA sample into a single strand.

2024年7月15日 · Explore the Maxam-Gilbert sequencing method and its pivotal role in advancing genomic research through detailed chemical and analytical techniques. Developed in the late 1970s, Maxam-Gilbert sequencing was among the pioneering techniques for …