The way to mitigate the impact of genetic drift on your mutant and transgenic mouse strain is to refresh the genetic background of your strains every 5-10 generations by backcrossing to the …

Backcrossing is a crossing of a hybrid with one of its parents or an individual genetically similar to its parent, to achieve offspring with a genetic identity closer to that of the parent. It is used in …

Backcross your strain ~10 times to your desired genetic background. A Genome Scan might help improve the speed at which this can be done, but may be limited in generating reliable data. …

2012年6月29日 · As a standard, when mice are back-crossed >8 times under standard conditions they care considered pure. At this point they can either be back-crossed further or intercrossed …

Our data demonstrate that high-speed backcrossing through the female germline is feasible and practical with small mouse numbers. Congenic mouse strains are defined as those in which a …

Backcrossing is a two-generation breeding protocol that starts by generating hybrid F1 mice between two inbred strains (preferably distantly related), one of them carrying the mutation of …

If the mutation cannot be propagated in the homozygous state, it will be maintained by continuous backcrossing of heterozygous animals to the original inbred strain. In both cases, the new …

2024年1月2日 · If your mutation is maintained on one genetic background but you wish to analyze it on another, it is a simple matter to transfer the mutation to a recipient strain background by …

2019年3月26日 · Genetically Engineered Mutant Mouse (GEMM) strains may be backcrossed to the appropriate inbred or hybrid mouse strain purchased from a reputable mouse repository or …

Our data demonstrate that high-speed backcrossing through the female germline is feasible and practical with small mouse numbers. Congenic mouse strains are defined as those in which a …