Plasmid transfection into mammalian cells is fairly straightforward and the resultant cells can either express the plasmid DNA transiently (similar to bacteria) or incorporate the genetic …

Gene transfer into mammalian cells may be effected either by infection with virus that carries the recombinant gene of interest, or by direct transfer of plasmid DNA. This chapter discusses the …

The different vector systems that transfer the gene into mammalian cells like plasmid based expression vectors, adenovirus vectors, vaccinia vectors, retroviral vector and baculovirus as …

We designed an inducible vector to regulate the expression of important genes in mammalian cells with five Lac operators to address the limitations related with previous systems.

2010年11月16日 · Current technologies for multigene expression in mammalian cells are inefficient, inflexible and time-consuming. In this paper we describe MultiLabel, a novel and …

2003年1月1日 · Gene transfer into mammalian cells may be effected either by infection with virus that carries the recombinant gene of interest, or by direct transfer of plasmid DNA. This …

In this paper we describe MultiLabel, a novel and highly eficient modular plasmid-based eukaryotic expression system. Independent expression vectors are assembled by a Cre/LoxP …

2010年11月16日 · Current technologies for multigene expression in mammalian cells are inefficient, inflexible and time-consuming. In this paper we describe MultiLabel, a novel and …

2023年4月18日 · We established a simple, universal direct cloning plasmid system for gene expression in both prokaryotic (E. coli) and mammalian cells. This system (pgMAX-II) can be …

2025年1月18日 · Plasmids are small, circular DNA molecules that exist independently of chromosomal DNA in bacteria and some eukaryotes. They are essential tools in molecular …