2017年1月30日 · Here, we present CRISPR–FLIP, a strategy that provides an efficient, rapid and scalable method for biallelic conditional gene knockouts in diploid or aneuploid cells, such as pluripotent stem...

Here, we present CRISPR-FLIP, a strategy that provides an efficient, rapid, and scalable method for bi-allelic conditional gene knockouts in diploid or aneuploid cells such as pluripotent stem cells, 3D organoids and cell lines by co-delivery of CRISPR/Cas9 and a …

Here, we present CRISPR-FLIP, a strategy that provides an efficient, rapid and scalable method for biallelic conditional gene knockouts in diploid or aneuploid cells, such as pluripotent stem cells, 3D organoids and cell lines, by co-delivery of CRISPR-Cas9 and …

In this study, we compared inducible CRISPR systems for gene knockout (using Cas9) or knockdown (using dCas9-KRAB) to enable temporal control over loss-of-function phenotypes in iPSCs and differentiated cell types.

Strategy for green fluorescent protein (GFP)-labeled reversible conditional gene knockout (cKO). This method requires a conventional floxed allele (Flox) paired with a multi-functional reversible KO (ΔR) allele (top left), and sequential action of Cre and Flp recombinases (middle and bottom).

2008年5月9日 · Inducible and reversible regulation of gene expression is a powerful approach for uncovering gene function. We have established a general method to efficiently produce reversible and inducible gene knockout and rescue in mice. In this system, which we named iKO, the target gene can be turned on and off at will by treating the mice with doxycycline.

One-step generation of conditional and reversible gene knockouts. Nat Methods. 2017 Jan 30. doi: 10.1038/nmeth.4156. PubMed Article. How was your experience today? Addgene is a nonprofit plasmid repository. We store and distribute high-quality plasmids from your colleagues.

2017年1月30日 · Here, we present CRISPR-FLIP, a strategy that provides an efficient, rapid and scalable method for biallelic conditional gene knockouts in diploid or aneuploid cells, such as pluripotent stem...

2022年9月3日 · To simply and tightly control temporal expression of the recombinases, on the basis of ReCOIN, we further presented a dual chemical-induced reversible gene knockout system (CIRKO) by insertion of...

4 天之前 · The DCAF16 gene is highly ... This study thus raises the provocative concept of incorporating electrophilic groups into reversible inhibitors ... A CRISPR–Cas9 knockout screen identified DCAF16 ...