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Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells (Video) | JoVE

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells (Video) | JoVE

已浏览 5834 次2020年3月3日

Plasmids 101: Origin of Replication

Plasmids 101: Origin of Replication

2017年2月28日

Label the image to review the process of gene cloning.SplicingandLigationRecombinantPlasmidPlasmidTranslationTranscriptionIsolated GeneGenetic DonorProtein ProductResetCloning starts with two main ingredients: the gene you are interested in (in this case, interferon, which has been cut out of its genome using appropriate restriction enzymes, and a cloning vector, which is usually a plasmid. Many different types of plasmids are available commercially.The first step in cloning is to prepare the is

Label the image to review the process of gene cloning.SplicingandLigationRecombinantPlasmidPlasmidTranslationTranscriptionIsolated GeneGenetic DonorProtein ProductResetCloning starts with two main ingredients: the gene you are interested in (in this case, interferon, which has been cut out of its genome using appropriate restriction enzymes, and a cloning vector, which is usually a plasmid. Many different types of plasmids are available commercially.The first step in cloning is to prepare the is

2024年1月9日

Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

2017年11月22日

Transcription/Translation

Transcription/Translation

2019年5月5日

An Introduction to DNA Transcription

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2017年3月8日

SOLVED: Often the orientation of the insert within the plasmid is critical, and only one orientation is desired. For example, if we needed to express the Spike gene using the pUC19-mini plasmid, it would be necessary to insert it such that the beginning of the gene ( 1, transcriptional start site) is adjacent to the promoter. In this instance, ensuring that only this orientation is possible would be extremely valuable and time-saving.Combine the large fragment of the PCR product containing the S

SOLVED: Often the orientation of the insert within the plasmid is critical, and only one orientation is desired. For example, if we needed to express the Spike gene using the pUC19-mini plasmid, it would be necessary to insert it such that the beginning of the gene ( 1, transcriptional start site) is adjacent to the promoter. In this instance, ensuring that only this orientation is possible would be extremely valuable and time-saving.Combine the large fragment of the PCR product containing the S

2021年10月20日

2.7 DNA replication, transcription and translation

2017年10月27日

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thermofisher.com

A linear plasmid contains only two genes, which are transcribed in opposite directions, each one from the end, toward the center of the plasmid. Draw diagrams of a. the plasmid DNA, showing the 5^' and 3^' ends of the nucleotide strands. b. the template strand for each gene. c. the positions of the transcription-initiation sites. d. the transcripts, showing the 5^' and 3^' ends. | Numerade

2020年6月7日

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2022年9月6日

The 5 Steps of Transcription From DNA to RNA

2017年3月8日

SOLVED: What information do you obtain from the double digest XhoI/AlwNI? What is your expectation if the plasmid is a reclosed vector without an insert? What is your expectation if your plasmid has an insert in the correct orientation (how many bands, what are their sizes)? What is your expectation if your plasmid has an insert in the incorrect orientation (how many bands, what are their sizes)?

2022年1月25日

Changing the Direction and Orientation of Electric Field During Electric Pulses Application Improves Plasmid Gene Transfer in vitro

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SOLVED:The promoter of an operon is the site to which RNA polymerase binds to begin transcription. Certain base changes in the promoter result in a mutant site to which RNA polymerase cannot bind. Would you expect mutations in the promoter that prevent binding of RNA polymerase to act in trans on another copy of the operon on a plasmid in the cell, or only in cis on the copy immediately adjacent to the mutated site?

2020年6月7日

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